Colorectal cancer is the third most common cancer in men and women worldwide. Almost 55% of cases occur in the more developed regions of the world. Recent scientific research suggests that a contributing factor could be excess iron in the intestine from a diet rich in red meat.
Iron is essential for our diet. For example, red blood cells contain iron and is crucial for oxygen transport around the body. However, too much iron in the large intestine is believed to cause the formation of tumours.
Chemists and cancer scientists working with Chris Tselepis at the University of Birmingham are looking at ways to ‘mop up’ excess iron in the large intestine. The aim is to develop a drug that will bind iron in the large intestine to prevent the onset of cancer.
Researchers are currently looking at a biological polymer (a biopolymer), called alginate. Polymers are very large molecules made up of lots of smaller, repeating, units. This biopolymer binds iron, and also meets many of the requirements needed for a drug to be active in the large intestine.
See ex. 1 in resource pack
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Alginates are an example of a structural biopolymer. Structural biopolymers are polymers that are produced by living organisms. They provide plants with their strong stems, crustaceans with their hard shells and our bodies with flexibility and movement.
Alginates are polymers made from several hundred sugar (saccharide) monomers. There are two types of monomer, known as M (β-D-mannuronate) and G (α-L-guluronate). The polymer is made up of blocks of identical or alternating monomers (MMMMMM, GGGGGG or MGMGMG). Different species of seaweed produce alginates with different composition.
Alginates are potential cancer prevention drugs, because they can bind iron. However, they also bind calcium really well, which needs to be absorbed by the body for use in teeth and bones.
Where there are sections of the alginate polymer with lots of ‘G’ segments, they bind both iron and calcium ions particularly strongly. We therefore do not want lots of ‘G‘ segments.
We need an alginate that will bind iron but not calcium (i.e. selective binding of iron). Therefore, researchers at the University of Birmingham are testing alginates that have lots of ‘M’ segments.
See ex. 2 in resource pack and here.
To observe the binding of 2 different alginates with solutions containing Ca(II) and Fe(III) ions, to determine which is most suited for an anti-cancer drug.
YOU WILL NEED
- 6 small beakers
- plastic pipettes
- solution ‘High G’ alginate (Manugel GHB) (aq) (plus food colouring)
- solution of ‘High M’ alginate (ProtaSea AFH, or Manucol LD ) (aq) (plus food colouring)
- 0.1% w/v CaCl2 (aq) solution
- 0.1% w/v FeCl3 (aq) solution
Comparing the binding of calcium ions: Pour 25 mL of CaCl2 solution into two beakers, and label them G and M. Using a plastic pipette, suck up 1-2 mL of the viscous ‘High G alginate’ solution, and pipette it dropwise into the CaCl2 solution, in the beaker marked G. Repeat this with the ‘High M alginate’, in beaker M, and observe the difference – swirl the beakers for a few seconds. You can tip the gels into your hand, over a sink, to see the differences.
Comparing the binding of iron ions: Pour 25 mL of FeCl3 solution into two beakers, and label them G and M. Do not get this solution on your hands as it is acidic and corrosive. Using a plastic pipette, suck up 1-2 mL of the viscous ‘High G alginate’ solution, and pipette it dropwise into the FeCl3 solution, in the beaker marked G. Repeat this with the ‘High M alginate’, in beaker M. Gently swirl the solutions for a few seconds -what happens in each case?
Control experiment: Pour 25 mL of deionised water into two beakers, and label them G and M. Using a plastic pipette, suck up 1-2 mL of the viscous ‘High G alginate’ solution, and pipette it dropwise into the beaker marked G. Repeat this with the ‘High M alginate’, in beaker M. Gently swirl the solutions for a few seconds – what do these two solutions look like?
For teachers ▼
If you wish to conduct this experiment just to see the gelling of the ‘High G’-type alginates, it can be adapted using Sodium Alginate purchased from a chemical supplier, such as sigma aldrich, or a specialist food supplier; http://www.souschef.co.uk/sodium-alginate.html. Make the alginate solution up, adding solid to stirring water, until a viscous, but pourable consistency is reached. (The amount of alginate needed to be added will vary, in the range 0.5-3 g per 100 mL of water). More information can be found here.
- What do the two alginates look like when they interact with calcium ions? Can you pick up the gel balls?
- Which alginate binds more strongly with calcium ions?
- What do the two alginates look like when they interact with iron ions?
- Look at the control solutions, where you have just mixed the alginates with water – what happens? What does this confirm?
- Which of the two alginates do you think will act best as a drug to bind iron, in a calcium-rich environment?
In the research lab
In the School of Cancer Sciences at the University of Birmingham, researchers, including Dr Richard Horniblow, working in the group of Dr Chris Tselepis, are currently testing a ‘high M‘ alginates in clinical trials. Read about this work in the Daily Mail article, and find out more about the group’s research here.
In the literature
Page author: Nicola Rogers Simpson
Edited 05/07/2022 Emily Hanover
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